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Methods Mol Biol. 2017;1566:109-124. doi: 10.1007/978-1-4939-6820-6_11.

Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function.

Author information

1
Metabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10021, USA.
2
Metabolic Health Center, Division of Cardiology, Department of Medicine, Weill Cornell Medicine, 413 East 69th Street, New York, NY, 10021, USA. jal2063@med.cornell.edu.

Abstract

Viral vectors have become widely used tools for genetic manipulation of adipose tissues to understand the biology and function of adipocytes in metabolism. There are a number of different viral vectors commonly used: retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). Here, we review examples from the literature and describe methods to transduce adipocytes and adipose tissues using retrovirus, lentivirus, adenovirus, and AAV to ascertain gene function in adipose biology.

KEYWORDS:

AAV; Adeno-associated virus; Adenovirus; Adipocyte; Adipogenesis; Adipokine; Adipose tissue; Beige fat; Brown fat; Diabetes; Lentivirus; Metabolism; Obesity; Retrovirus; Transduction; Viral vectors; White fat

PMID:
28244045
DOI:
10.1007/978-1-4939-6820-6_11
[Indexed for MEDLINE]

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