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Methods Mol Biol. 2017;1566:77-85. doi: 10.1007/978-1-4939-6820-6_8.

Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines.

Author information

1
Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, One Joslin Place, Boston, MA, 02215, USA.
2
Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. yu-hua.tseng@joslin.harvard.edu.

Abstract

Human brown and white preadipocytes offer unique cell models to study human adipogenesis and thermogenesis. Here, we describe the detailed procedures for isolation of human brown and white predipocytes from deep and superficial neck fat. To grow these cells in vitro for a prolonged period of time, they should be immortalized following the procedure discussed here. We also provide the protocol for expansion, cryopreservation, and adipogenic differentiation of cells.

KEYWORDS:

Brown and white adipose tissue; Differentiation; Immortalization; Neck fat; Preadipocytes; Stromal vascular fraction (SVF)

PMID:
28244042
PMCID:
PMC5870121
DOI:
10.1007/978-1-4939-6820-6_8
[Indexed for MEDLINE]
Free PMC Article

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