Format

Send to

Choose Destination
Intravital. 2016 Jul 25;5(3):e1215789. doi: 10.1080/21659087.2016.1215789. eCollection 2016.

An intravital microscopy model to study early pancreatic inflammation in type 1 diabetes in NOD mice.

Author information

1
Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada; Department of Pharmacology, Dalhousie University, Halifax, NS, Canada; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada; Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.
2
Department of Biology, Dalhousie University , Halifax, NS, Canada.
3
Department of Medicine, Dalhousie University , Halifax, NS, Canada.
4
Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.

Abstract

Intravital microscopy (IVM) of the pancreas has been proven to be an invaluable tool in pancreatitis, transplantation and ischemia/reperfusion research. Also in type 1 diabetes (T1D) pancreatic IVM offers unique advantages for the elucidation of the disease process. Female non-obese diabetic (NOD) mice develop T1D spontaneously by 40 weeks of age. Our goal was to establish an IVM-based method to study early pancreatic inflammation in NOD mice, which can be used to screen novel medications to prevent or delay T1D in future studies. This included evaluation of leukocyte-endothelial interactions as well as disturbances of capillary perfusion in the pancreatic microcirculation.

KEYWORDS:

Type 1 diabetes; functional capillary density; inflammation; intravital microscopy; leukocyte adherence

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center