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Gene. 1987;56(2-3):283-8.

A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis.

Author information

1
Institute of Medical Science, University of Tokyo, Japan.

Abstract

A series of variants of transposon Tn5 were constructed by replacement of the 2.7-kb central segment which encodes kanamycin resistance with various other resistance-coding genes: tetracycline, chloramphenicol, gentamicin, trimethoprim, streptomycin or ampicillin. A thermosensitive replication mutant of the broad-host-range transmissible plasmid R388 was also constructed for use as a suicide vector for the delivery of transposable elements.

PMID:
2824292
DOI:
10.1016/0378-1119(87)90145-4
[Indexed for MEDLINE]

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