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J Food Sci Technol. 2017 Jan;54(1):38-44. doi: 10.1007/s13197-016-2426-2. Epub 2017 Jan 13.

Soybean peptide fractions inhibit human blood, breast and prostate cancer cell proliferation.

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Department of Food Science, University of Arkansas, 2650 North Young Avenue, Fayetteville, AR 72704 USA.
Department of Poultry Science, University of Arkansas, 1260 W Maple St., Fayetteville, AR 72701 USA.
Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock, AR 72205 USA.
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 115 Plant Sciences Building, Fayetteville, AR 72701 USA.


In this study, we examined in vitro the bio-activity of peptide fractions obtained from soybeans against blood (CCRF-CEM and Kasumi-3), breast (MCF-7), and prostate (PC-3) cancer cell proliferation. Gastro-intestinal treated peptide fractions (<5, 5-10 and 10-50 kDa) prepared from seed proteins of two high oleic acid soybean lines-N98-4445A, S03-543CR and one high protein line-R95-1705, were tested for anticancer activity against human breast, blood and prostate cancer cell lines. Anti-proliferative cell titer assay was conducted to assess the inhibitory effects of the peptide fractions, while trypan blue dye exclusion assay was used to determine the dose response of most effective fractions. Results showed that the peptide fractions inhibited the cancer cell lines up to 68.0% and the minimum concentration to get 50% inhibitory activity (IC50) ranged between 608 and 678 µg/mL. This multiple site in vitro cancer inhibition by GI friendly peptides could have the potential use as food ingredients or nutritional supplements in an alternative cancer therapy.


Bioactive peptides; Cancer cell lines; Cytotoxicity; MTT assay; Trypan blue dye exclusion assay

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