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Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2195-E2204. doi: 10.1073/pnas.1616173114. Epub 2017 Feb 27.

Aberrant splicing in maize rough endosperm3 reveals a conserved role for U12 splicing in eukaryotic multicellular development.

Author information

1
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611.
2
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611.
3
Department of Biology, University of Florida, Gainesville, FL 32611.
4
Genetics Institute, University of Florida, Gainesville, FL 32611.
5
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611; settles@ufl.edu.

Abstract

RNA splicing of U12-type introns functions in human cell differentiation, but it is not known whether this class of introns has a similar role in plants. The maize ROUGH ENDOSPERM3 (RGH3) protein is orthologous to the human splicing factor, ZRSR2. ZRSR2 mutations are associated with myelodysplastic syndrome (MDS) and cause U12 splicing defects. Maize rgh3 mutants have aberrant endosperm cell differentiation and proliferation. We found that most U12-type introns are retained or misspliced in rgh3 Genes affected in rgh3 and ZRSR2 mutants identify cell cycle and protein glycosylation as common pathways disrupted. Transcripts with retained U12-type introns can be found in polysomes, suggesting that splicing efficiency can alter protein isoforms. The rgh3 mutant protein disrupts colocalization with a known ZRSR2-interacting protein, U2AF2. These results indicate conserved function for RGH3/ZRSR2 in U12 splicing and a deeply conserved role for the minor spliceosome to promote cell differentiation from stem cells to terminal fates.

KEYWORDS:

cell differentiation; maize endosperm; minor spliceosome; myelodysplastic syndrome; stem cell

PMID:
28242684
PMCID:
PMC5358371
DOI:
10.1073/pnas.1616173114
[Indexed for MEDLINE]
Free PMC Article

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