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Stem Cell Reports. 2017 Mar 14;8(3):576-588. doi: 10.1016/j.stemcr.2017.01.020. Epub 2017 Feb 24.

Genome Transfer Prevents Fragmentation and Restores Developmental Potential of Developmentally Compromised Postovulatory Aged Mouse Oocytes.

Author information

1
The New York Stem Cell Foundation Research Institute, New York, NY 10032, USA.
2
The New York Stem Cell Foundation Research Institute, New York, NY 10032, USA; Department of Pediatrics, Naomi Berrie Diabetes Center, Columbia University, New York, NY 10032, USA. Electronic address: de2220@cumc.columbia.edu.

Abstract

Changes in oocyte quality can have great impact on the developmental potential of early embryos. Here we test whether nuclear genome transfer from a developmentally incompetent to a developmentally competent oocyte can restore developmental potential. Using in vitro oocyte aging as a model system we performed nuclear transfer in mouse oocytes at metaphase II or at the first interphase, and observed that development to the blastocyst stage and to term was as efficient as in control embryos. The increased developmental potential is explained primarily by correction of abnormal cytokinesis at anaphase of meiosis and mitosis, by a reduction in chromosome segregation errors, and by normalization of the localization of chromosome passenger complex components survivin and cyclin B1. These observations demonstrate that developmental decline is primarily due to abnormal function of cytoplasmic factors involved in cytokinesis, while the genome remains developmentally fully competent.

KEYWORDS:

fragmentation; genome exchange; mouse; oocyte aging

PMID:
28242217
PMCID:
PMC5355644
DOI:
10.1016/j.stemcr.2017.01.020
[Indexed for MEDLINE]
Free PMC Article

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