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BMC Oral Health. 2017 Feb 27;17(1):57. doi: 10.1186/s12903-017-0348-7.

Response of human dental pulp cells to a silver-containing PLGA/TCP-nanofabric as a potential antibacterial regenerative pulp-capping material.

Author information

1
Department of Preventive, Restorative and Pediatric Dentistry, School of Dentistry, University of Bern, Bern, Switzerland.
2
Department of Conservative Dentistry & Periodontology, Medical University of Vienna, Vienna, Austria.
3
Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland.
4
Department of Periodontology, College of Dental Medicine, Nova Southeastern University, Fort Lauderdale, Florida, USA.
5
Robert K. Schenk Laboratory of Oral Histology, Department of Periodontology, Department of Oral Surgery and Stomatology, University of Bern, Bern, Switzerland.
6
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, Zurich, CH-8032, Switzerland.
7
Department of Preventive, Restorative and Pediatric Dentistry, School of Dentistry, University of Bern, Bern, Switzerland. patrick.schmidlin@zzm.uzh.ch.
8
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, Zurich, CH-8032, Switzerland. patrick.schmidlin@zzm.uzh.ch.

Abstract

BACKGROUND:

Damage or exposure of the dental pulp requires immediate therapeutic intervention.

METHODS:

This study assessed the biocompatibility of a silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro models, i.e. the material adapted on pre-cultured cells and cells directly cultured on the material, respectively. Collagen saffolds with and without hyaluronan acid (Coll-HA; Coll) using both cell culturing methods and cells growing on culture plates served as reference. Cell viability and proliferation were assessed after 24, 48, and 72 h based on formazan formation and BrdU incorporation. Scaffolds were harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha, and alkaline phosphatase (AP) was assessed 24 h after stimulation.

RESULTS:

In both models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on dental pulp cells, while no significant reduction was found in cells with Coll and Coll-HA. Cells with PLGA/Ag-TCP for 72 h showed similar relative BrdU incorporation than cells stimulated with Coll and Coll-HA. A prominent increase in the pro-inflammatory genes IL-6 and TNF-α was observed when cells were cultured with PLGA/Ag-TCP compared to the other groups. This increase was parallel with a slight increase in AP expression. Overall, no differences between the two culture methods were observed.

CONCLUSIONS:

PLGA/Ag-TCP decreased viability and proliferation rate of human dental pulp cells and increased the pro-inflammatory capacity and alkaline phosphatase expression. Whether these cellular responses observed in vitro translate into pulp regeneration in vivo will be assessed in further studies.

KEYWORDS:

Capping; Dental pulp; In vitro techniques; Regeneration

PMID:
28241819
PMCID:
PMC5327548
DOI:
10.1186/s12903-017-0348-7
[Indexed for MEDLINE]
Free PMC Article

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