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Nutrients. 2017 Feb 22;9(2). pii: E176. doi: 10.3390/nu9020176.

Age-Related Loss in Bone Mineral Density of Rats Fed Lifelong on a Fish Oil-Based Diet Is Avoided by Coenzyme Q10 Addition.

Author information

1
Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. alvarela@ugr.es.
2
Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. jjochoa@ugr.es.
3
Nuclear Medicine Service, Hospital Virgen de las Nieves, 18014 Granada, Spain. josem.llamas.sspa@juntadeandalucia.es.
4
Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. maglopez@ugr.es.
5
Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. elenamp@ugr.es.
6
Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Biochemistry and Molecular Biology II, Biomedical Research Center, University of Granada, 18100 Granada, Spain. mramirez@ugr.es.
7
Department of Pathology, Complejo Hospitalario de Jaén, 23007 Jaén, Spain. cesarl.ramirez.sspa@juntadeandalucia.es.
8
Department of Scienze Cliniche Specialistiche ed Odontostomatologiche, Università Politecnica delle Marche, 60131 Ancona, Italy. fgiampie@mta01.univpm.it.
9
Department of Scienze Cliniche Specialistiche ed Odontostomatologiche, Università Politecnica delle Marche, 60131 Ancona, Italy. m.a.battino@univpm.it.
10
Institute of Nutrition and Food Technology "José Mataix Verdú", Department of Physiology, Biomedical Research Center, University of Granada, 18100 Granada, Spain. jlquiles@ugr.es.

Abstract

During aging, bone mass declines increasing osteoporosis and fracture risks. Oxidative stress has been related to this bone loss, making dietary compounds with antioxidant properties a promising weapon. Male Wistar rats were maintained for 6 or 24 months on diets with fish oil as unique fat source, supplemented or not with coenzyme Q10 (CoQ10), to evaluate the potential of adding this molecule to the n-3 polyunsaturated fatty acid (n-3 PUFA)-based diet for bone mineral density (BMD) preservation. BMD was evaluated in the femur. Serum osteocalcin, osteopontin, receptor activator of nuclear factor-κB ligand, ostroprotegerin, parathyroid hormone, urinary F₂-isoprostanes, and lymphocytes DNA strand breaks were also measured. BMD was lower in aged rats fed a diet without CoQ10 respect than their younger counterparts, whereas older animals receiving CoQ10 showed the highest BMD. F₂-isoprostanes and DNA strand breaks showed that oxidative stress was higher during aging. Supplementation with CoQ10 prevented oxidative damage to lipid and DNA, in young and old animals, respectively. Reduced oxidative stress associated to CoQ10 supplementation of this n-3 PUFA-rich diet might explain the higher BMD found in aged rats in this group of animals.

KEYWORDS:

antioxidants; dietary fat; n-3 PUFA; oxidative stress; ubiquinone

PMID:
28241421
PMCID:
PMC5331607
DOI:
10.3390/nu9020176
[Indexed for MEDLINE]
Free PMC Article

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