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Sci Rep. 2017 Feb 27;7:43217. doi: 10.1038/srep43217.

Low-Cost Optical Mapping Systems for Panoramic Imaging of Complex Arrhythmias and Drug-Action in Translational Heart Models.

Author information

1
Essel Research and Development Inc., Toronto, Canada.
2
ITACA, Universitat Politècnica de València, Valencia, Spain.
3
Spanish National Cardiovascular Research Center, Carlos III (CNIC), Madrid, Spain.
4
Departments of Physiology and Cardiology, Universitat de València, Clinic Hospital - INCLIVA, Valencia, Spain.
5
Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (CIBERCV), Spain.
6
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), Madrid, Spain.
7
Richard D. Berlin Center for Cell Analysis and Modeling, University of Connecticut School of Medicine, Farmington, CT, USA.

Abstract

Panoramic optical mapping is the primary method for imaging electrophysiological activity from the entire outer surface of Langendorff-perfused hearts. To date, it is the only method of simultaneously measuring multiple key electrophysiological parameters, such as transmembrane voltage and intracellular free calcium, at high spatial and temporal resolution. Despite the impact it has already had on the fields of cardiac arrhythmias and whole-heart computational modeling, present-day system designs precludes its adoption by the broader cardiovascular research community because of their high costs. Taking advantage of recent technological advances, we developed and validated low-cost optical mapping systems for panoramic imaging using Langendorff-perfused pig hearts, a clinically-relevant model in basic research and bioengineering. By significantly lowering financial thresholds, this powerful cardiac electrophysiology imaging modality may gain wider use in research and, even, teaching laboratories, which we substantiated using the lower-cost Langendorff-perfused rabbit heart model.

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