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Curr Biol. 2017 Mar 6;27(5):624-637. doi: 10.1016/j.cub.2017.01.033. Epub 2017 Feb 23.

Coordination by Cdc42 of Actin, Contractility, and Adhesion for Melanoblast Movement in Mouse Skin.

Author information

1
CRUK Beatson Institute, University of Glasgow, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
2
Division of Molecular Cell Biology, Zoological Institute, Technische Universität Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany; Molecular Cell Biology Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
3
Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27514, USA.
4
Institute Curie, CNRS UMR3347, INSERM U1021, Bat 110, Centre Universitaire, 91405 Orsay Cedex, France.
5
Biotech Research Center, University of Copenhagen, Ole Maaløes Vej 5, Copenhagen 2200, Denmark.
6
CRUK Beatson Institute, University of Glasgow, Switchback Road, Bearsden, Glasgow G61 1BD, UK. Electronic address: l.machesky@beatson.gla.ac.uk.

Abstract

The individual molecular pathways downstream of Cdc42, Rac, and Rho GTPases are well documented, but we know surprisingly little about how these pathways are coordinated when cells move in a complex environment in vivo. In the developing embryo, melanoblasts originating from the neural crest must traverse the dermis to reach the epidermis of the skin and hair follicles. We previously established that Rac1 signals via Scar/WAVE and Arp2/3 to effect pseudopod extension and migration of melanoblasts in skin. Here we show that RhoA is redundant in the melanocyte lineage but that Cdc42 coordinates multiple motility systems independent of Rac1. Similar to Rac1 knockouts, Cdc42 null mice displayed a severe loss of pigmentation, and melanoblasts showed cell-cycle progression, migration, and cytokinesis defects. However, unlike Rac1 knockouts, Cdc42 null melanoblasts were elongated and displayed large, bulky pseudopods with dynamic actin bursts. Despite assuming an elongated shape usually associated with fast mesenchymal motility, Cdc42 knockout melanoblasts migrated slowly and inefficiently in the epidermis, with nearly static pseudopods. Although much of the basic actin machinery was intact, Cdc42 null cells lacked the ability to polarize their Golgi and coordinate motility systems for efficient movement. Loss of Cdc42 de-coupled three main systems: actin assembly via the formin FMNL2 and Arp2/3, active myosin-II localization, and integrin-based adhesion dynamics.

KEYWORDS:

Cdc42; Rho GTPases; actin; actin cytoskeleton; adhesion; cell motility; integrin; melanocyte; migration; myosin

PMID:
28238662
PMCID:
PMC5344686
DOI:
10.1016/j.cub.2017.01.033
[Indexed for MEDLINE]
Free PMC Article

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