Format

Send to

Choose Destination
Neurobiol Dis. 2017 Jun;102:49-59. doi: 10.1016/j.nbd.2017.02.005. Epub 2017 Feb 24.

Preventive motor training but not progenitor grafting ameliorates cerebellar ataxia and deregulated autophagy in tambaleante mice.

Author information

1
Department of Neuroscience Rita Levi-Montalcini, University of Turin, Italy; Neuroscience Institute Cavalieri Ottolenghi, Regione Gonzole, 10043 Orbassano, Turin, Italy. Electronic address: elisa.fuca@unito.it.
2
Neuroscience Institute Cavalieri Ottolenghi, Regione Gonzole, 10043 Orbassano, Turin, Italy.
3
Department of Neuroscience Rita Levi-Montalcini, University of Turin, Italy; Neuroscience Institute Cavalieri Ottolenghi, Regione Gonzole, 10043 Orbassano, Turin, Italy.
4
Department of Neuroscience Rita Levi-Montalcini, University of Turin, Italy.
5
Department of Neuroscience Rita Levi-Montalcini, University of Turin, Italy; Neuroscience Institute Cavalieri Ottolenghi, Regione Gonzole, 10043 Orbassano, Turin, Italy. Electronic address: annalisa.buffo@unito.it.

Abstract

Treatment options for degenerative cerebellar ataxias are currently very limited. A large fraction of such disorders is represented by hereditary cerebellar ataxias, whose familiar transmission facilitates an early diagnosis and may possibly allow to start preventive treatments before the onset of the neurodegeneration and appearance of first symptoms. In spite of the heterogeneous aetiology, histological alterations of ataxias often include the primary degeneration of the cerebellar cortex caused by Purkinje cells (PCs) loss. Thus, approaches aimed at replacing or preserving PCs could represent promising ways of disease management. In the present study, we compared the efficacy of two different preventive strategies, namely cell replacement and motor training. We used tambaleante (tbl) mice as a model for progressive ataxia caused by selective loss of PCs and evaluated the effectiveness of the preventive transplantation of healthy PCs into early postnatal tbl cerebella, in terms of PC replacement and functional preservation. On the other hand, we investigated the effects of motor training on PC survival, cerebellar circuitry and their behavioral correlates. Our results demonstrate that, despite a good survival rate and integration of grafted PCs, the adopted grafting protocol could not alleviate the ataxic symptoms in tbl mice. Conversely, preventive motor training increases PCs survival with a moderate positive impact on the motor phenotype.

KEYWORDS:

Mouse; Neurodegeneration; Neuroprotection; Neurotransplantation; Purkinje neurons

PMID:
28237314
DOI:
10.1016/j.nbd.2017.02.005
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science Icon for University of Turin Instituional Repository AperTO
Loading ...
Support Center