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Anal Chem. 2017 Mar 21;89(6):3501-3507. doi: 10.1021/acs.analchem.6b04596. Epub 2017 Mar 7.

Label-Free Method Using a Weighted-Phase Algorithm To Quantitate Nanoscale Interactions between Molecules on DNA Microarrays.

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Department of Biomedical Engineering, Tsinghua University School of Medicine , Beijing 100084, PR China.
National Engineering Research Center for Beijing Biochip Technology , Beijing 102206, PR China.


White light interference is used as a label-free method to detect nanoscale changes on surfaces. However, the signal-to-noise ratio of the white light interference method is very low, thus resulting in inaccurate results. In this paper, we report a corrected label-free method based on hyperspectral interferometry to overcome the shortcoming of the white light interference method. A platform based on hyperspectral interferometry was established, and a DNA hybridization microarray was constructed to quantitate thickness variation of molecules on a solid surface. We used fluorescence resonance energy transfer (FRET) to validate the results of our method. Compared to conventional fluorescence-labeled method like FRET, our method has advantages because it does not require a fluorescent label and has a detection limit of 1.78 nm, a high accuracy, and wide detection range (5-64 bp).

[Indexed for MEDLINE]

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