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Nutrients. 2017 Feb 20;9(2). pii: E165. doi: 10.3390/nu9020165.

Catechol Groups Enable Reactive Oxygen Species Scavenging-Mediated Suppression of PKD-NFkappaB-IL-8 Signaling Pathway by Chlorogenic and Caffeic Acids in Human Intestinal Cells.

Author information

1
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. hsshin@kfri.re.kr.
2
Division of Nutrition and Metabolism Research, Korea Food Research Institute, Seongnam-si 13539, Korea. hsshin@kfri.re.kr.
3
Department of Food Biotechnology, University of Science and Technology (UST), Daejeon 34113, Korea. hsshin@kfri.re.kr.
4
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. satsu@maebashi-it.ac.jp.
5
Department of Biotechnology, Faculty of Engineering, Maebashi Institute of Technology, Gunma 371-0816, Japan. satsu@maebashi-it.ac.jp.
6
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. japanpaper04@gmail.com.
7
Institutes of Entrepreneurial BioConvergence, Seoul National University, Seoul 08826, Korea. japanpaper04@gmail.com.
8
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. atotuka@mail.ecc.u-tokyo.ac.jp.
9
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan. ms205346@nodai.ac.jp.
10
Department of Nutritional Science, Tokyo University of Agriculture, Tokyo 156-8502, Japan. ms205346@nodai.ac.jp.

Abstract

Chlorogenic acid (CHA) and caffeic acid (CA) are phenolic compounds found in coffee, which inhibit oxidative stress-induced interleukin (IL)-8 production in intestinal epithelial cells, thereby suppressing serious cellular injury and inflammatory intestinal diseases. Therefore, we investigated the anti-inflammatory mechanism of CHA and CA, both of which inhibited hydrogen peroxide (H₂O₂)-induced IL-8 transcriptional activity. They also significantly suppressed nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) transcriptional activity, nuclear translocation of the p65 subunit, and phosphorylation of IκB kinase (IKK). Additionally, upstream of IKK, protein kinase D (PKD) was also suppressed. Finally, we found that they scavenged H₂O₂-induced reactive oxygen species (ROS) and the functional moiety responsible for the anti-inflammatory effects of CHA and CA was the catechol group. Therefore, we conclude that the presence of catechol groups in CHA and CA allows scavenging of intracellular ROS, thereby inhibiting H₂O₂-induced IL-8 production via suppression of PKD-NF-κB signaling in human intestinal epithelial cells.

KEYWORDS:

caffeic acid; catechol group; chlorogenic acid; interleukine-8; nuclear factor κB; protein kinase D; reactive oxygen species

PMID:
28230729
PMCID:
PMC5331596
DOI:
10.3390/nu9020165
[Indexed for MEDLINE]
Free PMC Article

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