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J Agric Food Chem. 2017 Mar 15;65(10):2089-2099. doi: 10.1021/acs.jafc.7b00095. Epub 2017 Mar 1.

10-Gingerol, a Phytochemical Derivative from "Tongling White Ginger", Inhibits Cervical Cancer: Insights into the Molecular Mechanism and Inhibitory Targets.

Author information

1
School of Food Science and Engineering, Hefei University of Technology , Hefei 230009, People's Republic of China.
2
Agricultural and Forestry Specialty Food Processing Industry Technological Innovation Strategic Alliance of Anhui Province , Hefei 230009, People's Republic of China.

Abstract

With the aim of evaluating anticancerous activities of 10-gingerol (10-G) against HeLa cells, it was purified and identified from "Tongling white ginger" by HSCCC, UPLC-TOF-MS/MS, and NMR analysis, respectively. 10-G inhibited the proliferation of HeLa cells at IC50 (29.19 μM) and IC80 (50.87 μM) with altered cell morphology, increased cytotoxicity, and arrested cell cycle in the G0/G1 phase. Most cell cycle related genes and protein expression significantly decreased, followed by a slight decrease in a few without affecting cyclin B1 and cyclin E1 (protein). Both death receptors significantly up-regulated and activated apoptosis indicators (caspase family). Furthermore, significant changes in mitochondria-dependent pathway markers were observed and led to cell death. 10-G led to PI3K/AKT inhibition and AMPK activation to induce mTOR-mediated cell apoptosis in HeLa cells. These results can be an asset to exploit 10-G with other medicinal plant derivatives for future applications.

KEYWORDS:

10-gingerol; HeLa cells; PI3K/AKT/AMPK/mTOR pathways; Tongling white ginger; apoptosis

PMID:
28230361
DOI:
10.1021/acs.jafc.7b00095
[Indexed for MEDLINE]

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