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BMC Med. 2017 Feb 23;15(1):37. doi: 10.1186/s12916-017-0784-x.

Relationship between salivary/pancreatic amylase and body mass index: a systems biology approach.

Author information

1
University of Lille, CNRS, Institut Pasteur de Lille, UMR 8199 - EGID, Lille, 59000, France. amelie.bonnefond@inserm.fr.
2
University of Lille, CNRS, Institut Pasteur de Lille, UMR 8199 - EGID, Lille, 59000, France.
3
Institute for Molecular Bioscience, The University of Queensland, Brisbane, 4067, Australia.
4
University of Lille, Inserm, U1190 - EGID, Lille, 59000, France.
5
Endocrine Surgery Department, CHU of Lille, Lille, 59000, France.
6
Qatar Biomedical Research Institute, Qatar Foundation, Doha, 5825, Qatar.
7
Fleurbaix-Laventie Association, Laventie, 62840, France.
8
Inserm CIE 05 - Department of Clinical Epidemiology, Robert Debré Hospital, Paris, 75019, France.
9
Pediatric Endocrine Department, CHU of Lille, Lille, 59000, France.
10
Inserm, U1138, Centre de Recherche des Cordeliers, Paris, 75006, France.
11
Paris-Diderot University, Sorbonne Paris-Cité, Paris, 75013, France.
12
Department of Endocrinology-Diabetology and Nutrition, DHU-FIRE, Bichat Hospital, Assistance Publique-Hôpitaux de Paris, Paris, 75018, France.
13
Inserm, U1018, CESP, Team 5 (EpReC, Renal and cardiovascular Epidemiology), UVSQ-UPS, Villejuif, 94807, France.
14
UF8832 - Biochimie Automatisée, Pôle de Biologie Pathologie Génétique, CHU of Lille, Lille, 59000, France.
15
University of Lille, CNRS, Institut Pasteur de Lille, UMR 8199 - EGID, Lille, 59000, France. froguel@good.ibl.fr.
16
Department of Genomics of Common Disease, School of Public Health, Imperial College London, Hammersmith Hospital, London, W12 0NN, UK. froguel@good.ibl.fr.

Abstract

BACKGROUND:

Salivary (AMY1) and pancreatic (AMY2) amylases hydrolyze starch. Copy number of AMY1A (encoding AMY1) was reported to be higher in populations with a high-starch diet and reduced in obese people. These results based on quantitative PCR have been challenged recently. We aimed to re-assess the relationship between amylase and adiposity using a systems biology approach.

METHODS:

We assessed the association between plasma enzymatic activity of AMY1 or AMY2, and several metabolic traits in almost 4000 French individuals from D.E.S.I.R. longitudinal study. The effect of the number of copies of AMY1A (encoding AMY1) or AMY2A (encoding AMY2) measured through droplet digital PCR was then analyzed on the same parameters in the same study. A Mendelian randomization analysis was also performed. We subsequently assessed the association between AMY1A copy number and obesity risk in two case-control studies (5000 samples in total). Finally, we assessed the association between body mass index (BMI)-related plasma metabolites and AMY1 or AMY2 activity.

RESULTS:

We evidenced strong associations between AMY1 or AMY2 activity and lower BMI. However, we found a modest contribution of AMY1A copy number to lower BMI. Mendelian randomization identified a causal negative effect of BMI on AMY1 and AMY2 activities. Yet, we also found a significant negative contribution of AMY1 activity at baseline to the change in BMI during the 9-year follow-up, and a significant contribution of AMY1A copy number to lower obesity risk in children, suggesting a bidirectional relationship between AMY1 activity and adiposity. Metabonomics identified a BMI-independent association between AMY1 activity and lactate, a product of complex carbohydrate fermentation.

CONCLUSIONS:

These findings provide new insights into the involvement of amylase in adiposity and starch metabolism.

KEYWORDS:

AMY1A/AMY2A; Body mass index; Copy number variant; Mendelian randomization; Metabonomics; Obesity; Salivary/Pancreatic amylase; Starch

PMID:
28228143
PMCID:
PMC5322607
DOI:
10.1186/s12916-017-0784-x
[Indexed for MEDLINE]
Free PMC Article

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