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Arch Endocrinol Metab. 2017 Sept-Oct;61(5):438-446. doi: 10.1590/2359-3997000000254. Epub 2017 Feb 16.

IL-6, TNF-α, and IL-10 levels/polymorphisms and their association with type 2 diabetes mellitus and obesity in Brazilian individuals.

Author information

1
Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brasil.
2
Instituto de Ensino e Pesquisa, Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brasil.
3
Faculdade de Farmácia, UFMG, Belo Horizonte, MG, Brasil.
4
Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Botucatu, SP, Brasil.

Abstract

OBJECTIVE:

This study aimed to investigate the association of plasma TNF-α, IL-6, and lL-10 levels and cytokine gene polymorphisms [TNF-α (-308 G→A), IL-6 (-174 C→G) and IL-10 (-1082 A→G, -819 T→C and -592 A→C)] in type 2 diabetes mellitus (T2DM) and obese patients.

SUBJECTS AND METHODS:

One hundred and two T2DM patients and 62 controls were included in this study. Cytokine plasma levels were measured by the Cytometric Bead Array method. Genotyping was carried out by the polymerase chain reaction.

RESULTS:

IL-6 levels were significantly different between T2DM patients and controls. Interestingly, IL-6 levels were higher in T2DM patients with BMI > 30 kg/m2 compared with other patients and obese controls. The genotype and allele frequencies were similar between patients and controls. In the T2DM group, the SNP IL-10 -819 T/C showed a difference between the cytokine level and genotypes: IL-10 level in the TT genotype was significantly higher when compared to CC genotype.

CONCLUSIONS:

These results suggest an association between IL-6 levels and obesity, and IL-10 levels and the SNP -819 T/C in T2DM. Knowledge of these variants in T2DM might contribute to a better understanding of the role of inflammation in the etiology and progression of this disease.

PMID:
28225860
DOI:
10.1590/2359-3997000000254
[Indexed for MEDLINE]
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