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Hepatol Res. 2017 Dec;47(13):1383-1389. doi: 10.1111/hepr.12878. Epub 2017 Mar 24.

25-Hydroxy vitamin D suppresses hepatitis C virus replication and contributes to rapid virological response of treatment efficacy.

Huang JF1,2,3, Ko YM1, Huang CF1,3,4, Yeh ML1,3, Dai CY1,2,3, Hsieh MH1, Huang CI1, Yang HL1, Wang SC1, Lin ZY1,3, Chen SC1,3, Yu ML1,2,3, Chuang WL1,2,3.

Author information

1
Hepatobiliary Division, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
2
Graduate Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
3
Faculty of Internal Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
4
Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.

Abstract

AIM:

25-Hydroxy vitamin D (Vit D) plays a role in treatment outcomes in chronic hepatitis C virus (HCV) infection. We aimed to clarify whether HCV replication is inhibited by Vit D in HCV replicon cells. Clinical implication was assessed for rapid virological response (RVR) and sustained virological response (SVR) among those patients receiving antiviral therapy.

METHODS:

Cell survival and viral loads were observed in Con1 (genotype 1b) and J6/JFH (genotype 2a) cells treated with different doses of Vit D. Three groups of patients with different treatment responses were recruited to assess their Vit D levels: group A, RVR-/SVR-; group B, RVR+/SVR-; and group C, RVR+/SVR+.

RESULTS:

The viral load of Con1 cells decreased by 69%, 80%, and 86% following treatment with 1 μM, 5 μM, and 10 μM Vit D, respectively (P < 0.0001). In J6/JFH cells, it decreased by 12%, 55%, and 80.5% following treatment with 1 μM, 5 μM, and 10 μM Vit D, respectively (P < 0.0001). There was a significant increase of Vit D between chronic hepatitis C groups, ranging from 4.4 ± 5.6 ng/mL in group A (n = 44), to 17.2 ± 11.6 ng/mL in group B (n = 44), and 32.5 ± 37.5 ng/mL of group C (n = 44) (P < 0.001). Advanced fibrosis (odds ratio = 0.13, 95% confidence interval = 0.04-0.41, P < 0.001) and Vit D deficiency (<10 ng/mL) (odds ratio = 0.11, 95% confidence interval = 0.03-0.43, P = 0.001) were predictive of SVR in the multivariate regression analysis.

CONCLUSION:

Vitamin D decreases HCV replication and also contributes to early treatment viral kinetics.

KEYWORDS:

25-hydroxy vitamin D; hepatitis C virus; replicon; viral kinetics

PMID:
28225575
DOI:
10.1111/hepr.12878

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