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Appl Plant Sci. 2017 Feb 7;5(2). pii: apps.1600126. doi: 10.3732/apps.1600126. eCollection 2017 Feb.

Identification and characterization of microsatellite markers in Pinus kesiya var. langbianensis (Pinaceae).

Author information

1
Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China; Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
2
Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.
3
Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, College of Forestry, Southwest Forestry University, Kunming, Yunnan 650224, People's Republic of China.

Abstract

PREMISE OF THE STUDY:

Microsatellite primers were developed in Pinus kesiya var. langbianensis (Pinaceae), a species native to southwestern China, to investigate its genetic diversity and population structure in order to provide information for the conservation and management of this species.

METHODS AND RESULTS:

Using next-generation sequencing, a total of 2349 putative simple sequence repeat primer pairs were designed. Eighteen polymorphic markers in 60 individuals belonging to four populations of P. kesiya var. langbianensis were identified and characterized with two to 11 alleles per locus. The observed and expected heterozygosity ranged from 0.000 to 0.800 and 0.000 to 0.840, respectively. Each of these loci cross-amplified in the closely related species P. massoniana, P. densata, P. tabuliformis, and P. yunnanensis, with one to seven alleles per locus.

CONCLUSIONS:

The new markers are promising tools to study the population genetics of P. kesiya var. langbianensis and related species.

KEYWORDS:

Pinaceae; Pinus kesiya var. langbianensis; microsatellite; next-generation sequencing; population genetics

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