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Plant Cell. 2017 Mar;29(3):589-599. doi: 10.1105/tpc.16.00769. Epub 2017 Feb 21.

IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis.

Author information

1
Peking University-Tsinghua University-National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing 100871, China.
2
National Institute of Biological Sciences, Beijing 102206, China.
3
Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
4
Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
5
Bionova (Beijing) Biotech Co., Beijing 102206, China.
6
Tsinghua-Peking Center for Life Sciences, Beijing 100084, China.
7
Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China qiyijun@tsinghua.edu.cn.

Abstract

Repair of DNA double-strand breaks (DSBs) is critical for the maintenance of genome integrity. We previously showed that DSB-induced small RNAs (diRNAs) facilitate homologous recombination-mediated DSB repair in Arabidopsis thaliana Here, we show that INVOLVED IN DE NOVO2 (IDN2), a double-stranded RNA binding protein involved in small RNA-directed DNA methylation, is required for DSB repair in Arabidopsis. We find that IDN2 interacts with the heterotrimeric replication protein A (RPA) complex. Depletion of IDN2 or the diRNA binding ARGONAUTE2 leads to increased accumulation of RPA at DSB sites and mislocalization of the recombination factor RAD51. These findings support a model in which IDN2 interacts with RPA and facilitates the release of RPA from single-stranded DNA tails and subsequent recruitment of RAD51 at DSB sites to promote DSB repair.

PMID:
28223440
PMCID:
PMC5385954
DOI:
10.1105/tpc.16.00769
[Indexed for MEDLINE]
Free PMC Article

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