Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin

Guilherme Guerra Alves; Ricardo Andrez Machado-de-Ávila; Carlos Delfin Chávez-Olórtegui; Rodrigo Otávio Silveira Silva; Francisco Carlos Faria Lobato

doi:10.1016/j.bjm.2016.10.023

Mapping of the continuous epitopes displayed on the Clostridium perfringens type D epsilon-toxin

Braz J Microbiol. 2017 Jul-Sep;48(3):570-575. doi: 10.1016/j.bjm.2016.10.023. Epub 2017 Feb 3.

Authors

Guilherme Guerra Alves¹, Ricardo Andrez Machado-de-Ávila², Carlos Delfin Chávez-Olórtegui³, Rodrigo Otávio Silveira Silva⁴, Francisco Carlos Faria Lobato⁴

Affiliations

¹ Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária, Belo Horizonte, MG, Brazil. Electronic address: guilhermeguerra.vet@gmail.com.
² Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Belo Horizonte, MG, Brazil; Universidade do Extremo Sul Catarinense (UNESC), Criciúma, SC, Brazil.
³ Universidade Federal de Minas Gerais (UFMG), Instituto de Ciências Biológicas, Belo Horizonte, MG, Brazil.
⁴ Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária, Belo Horizonte, MG, Brazil.

Abstract

The epsilon toxin, produced by Clostridium perfringens, is responsible for enterotoxemia in ruminants and is a potential bioterrorism agent. In the present study, 15 regions of the toxin were recognized by antibodies present in the serum, with different immunodominance scales, and may be antigen determinants that can be used to formulate subunit vaccines.

Keywords: Enterotoxemia; Epitope; Epsilon; Mapping; Toxin.

MeSH terms

Animals
Bacterial Toxins / chemistry*
Bacterial Toxins / genetics
Bacterial Toxins / immunology
Clostridium perfringens / chemistry
Clostridium perfringens / genetics
Clostridium perfringens / immunology*
Enterotoxemia / microbiology
Epitope Mapping
Epitopes / chemistry*
Epitopes / genetics
Epitopes / immunology

Substances

Bacterial Toxins
Epitopes