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BMC Infect Dis. 2017 Feb 22;17(1):166. doi: 10.1186/s12879-017-2258-3.

Meta-analysis of the utility of culture, biopsy, and direct KOH examination for the diagnosis of onychomycosis.

Author information

1
Biological Research Corporation (Corporación para Investigaciones Biológicas - CIB), Medellín, Colombia.
2
Experimental Medical Mycology Research Group, CIB, Medellín, Colombia.
3
School of Microbiology, University of Antioquia (Universidad de Antioquia), Calle 67 Número 53 - 108, Bloque 5, oficina 103, Medellín, Colombia. jaiberthcardona@gmail.com.
4
Faculty of Medicine, Cooperative University of Colombia (Universidad Cooperativa de Colombia), Medellín, Colombia. jaiberthcardona@gmail.com.

Abstract

BACKGROUND:

Onychomycosis is a highly prevalent disease worldwide. There is no standard test for its diagnosis, which remains costly, wasteful, and is sometimes delayed. The diagnostic tests for this disease are few and discordant. The objective was to evaluate the diagnostic validity, performance, and accuracy of culture, nail clipping with Periodic Acid-Schiff -PAS- staining (biopsy), and direct potassium hydroxide (KOH) examination for the study of onychomycosis.

METHODS:

A systematic review was conducted via meta-analysis using 5 databases and 21 search strategies. An ex ante protocol was applied with inclusion and exclusion criteria. Quality was assessed with the Quality Assessment of Diagnostic Accuracy Studies (QUADAS) tool, and the sensitivity, specificity, predictive values, likelihood ratios, diagnostic odds ratios, receiver operating characteristic (ROC) curves, and proportion of correctly diagnosed patients were evaluated with the meta-analysis of studies of evaluations of diagnostic and screening tests (Meta-DiSc) and Epidat using a random effects model.

RESULTS:

The efficiency or accuracy of the three tests is influenced by the methodological quality of the studies. These values are lower for KOH and culture and higher for biopsy in moderate quality studies.

CONCLUSION:

The diagnostic tests evaluated in this meta-analysis independently showed acceptable validity, performance, and efficiency, with nail clipping with PAS staining outperforming the other two tests.

KEYWORDS:

Diagnosis; Meta-analysis as topic; Onychomycosis; Test validity; Validation studies

PMID:
28222676
PMCID:
PMC5320683
DOI:
10.1186/s12879-017-2258-3
[Indexed for MEDLINE]
Free PMC Article

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