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BMC Complement Altern Med. 2017 Feb 20;17(1):117. doi: 10.1186/s12906-017-1637-z.

Bacopa monnieri extract increases rat coronary flow and protects against myocardial ischemia/reperfusion injury.

Author information

1
Department of Physiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
2
Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
3
PEPITE EA4267, University Bourgogne Franche-Comté, Besançon, F-25000, France.
4
Department of Medical Technology, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
5
Biomedical Research Unit in Cardiovascular Sciences, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
6
Department of Physiology, University of Bern, Bühlplatz 5, Bern, CH-3012, Switzerland.
7
Department of Physiology and Pathophysiology, Heidelberg University, Heidelberg, 69120, Germany.
8
Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, 65000, Thailand.
9
School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand.
10
Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand. krongkarnc@nu.ac.th.

Abstract

BACKGROUND:

This study explored Bacopa monnieri, a medicinal Ayurvedic herb, as a cardioprotectant against ischemia/reperfusion injury using cardiac function and coronary flow as end-points.

METHODS:

In normal isolated rat hearts, coronary flow, left ventricular developed pressure, heart rate, and functional recovery were measured using the Langendorff preparation. Hearts were perfused with either (i) Krebs-Henseleit (normal) solution, (control), or with 30, 100 μg/ml B. monnieri ethanolic extract (30 min), or (ii) with normal solution or extract for 10 min preceding no-perfusion ischemia (30 min) followed by reperfusion (30 min) with normal solution. Infarct volumes were measured by triphenyltetrazolium staining. L-type Ca2+-currents (ICa, L) were measured by whole-cell patching in HL-1 cells, a mouse atrial cardiomyocyte cell line. Cytotoxicity of B. monnieri was assessed in rat isolated ventricular myocytes by trypan blue exclusion.

RESULTS:

In normally perfused hearts, B. monnieri increased coronary flow by 63 ± 13% (30 μg/ml) and 216 ± 21% (100 μg/ml), compared to control (5 ± 3%) (n = 8-10, p < 0.001). B. monnieri treatment preceding ischemia/reperfusion improved left ventricular developed pressure by 84 ± 10% (30 μg/ml), 82 ± 10% (100 μg/ml) and 52 ± 6% (control) compared to pre- ischemia/reperfusion. Similarly, functional recovery showed a sustained increase. Moreover, B. monnieri (100 μg/ml) reduced the percentage of infarct size from 51 ± 2% (control) to 25 ± 2% (n = 6-8, p < 0.0001). B. monnieri (100 μg/ml) reduced ICa, L by 63 ± 4% in HL-1 cells. Ventricular myocyte survival decreased at higher concentrations (50-1000 μg/ml) B. monnieri.

CONCLUSIONS:

B. monnieri improves myocardial function following ischemia/reperfusion injury through recovery of coronary blood flow, contractile force and decrease in infarct size. Thus this may lead to a novel cardioprotectant strategy.

KEYWORDS:

Bacopa monnieri; Brahmi; Cardiac function; Coronary blood flow; Heart; Ischemia/reperfusion; Myocardial infarction

PMID:
28219356
PMCID:
PMC5319078
DOI:
10.1186/s12906-017-1637-z
[Indexed for MEDLINE]
Free PMC Article

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