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Nat Chem Biol. 2017 Apr;13(4):363-365. doi: 10.1038/nchembio.2314. Epub 2017 Feb 20.

Engineering fatty acid synthases for directed polyketide production.

Author information

1
Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence "Macromolecular Complexes," Goethe University Frankfurt, Frankfurt am Main, Germany.
2
Max Planck Institute for Biophysical Chemistry, Department of Theoretical and Computational Biophysics, Göttingen, Germany.
3
Max Planck Institute of Biochemistry, Project Group Biological Chemistry, Martinsried, Germany.

Abstract

In this study, we engineered fatty acid synthases (FAS) for the biosynthesis of short-chain fatty acids and polyketides, guided by a combined in vitro and in silico approach. Along with exploring the synthetic capability of FAS, we aim to build a foundation for efficient protein engineering, with the specific goal of harnessing evolutionarily related megadalton-scale polyketide synthases (PKS) for the tailored production of bioactive natural compounds.

PMID:
28218912
DOI:
10.1038/nchembio.2314
[Indexed for MEDLINE]

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