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Nat Chem Biol. 2017 Apr;13(4):360-362. doi: 10.1038/nchembio.2301. Epub 2017 Feb 20.

Expanding the product portfolio of fungal type I fatty acid synthases.

Zhu Z1,2,3, Zhou YJ1,3, Krivoruchko A1,3,4, Grininger M5, Zhao ZK2,6, Nielsen J1,3,4,7,8.

Author information

1
Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
2
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
3
Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, Gothenburg, Sweden.
4
Biopetrolia AB, Systems and Synthetic Biology, Chalmers University of Technology, Gothenburg, Sweden.
5
Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Cluster of Excellence for Macromolecular Complexes, Goethe University Frankfurt, Frankfurt am Main, Germany.
6
Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian, China.
7
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
8
Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden.

Abstract

Fungal type I fatty acid synthases (FASs) are mega-enzymes with two separated, identical compartments, in which the acyl carrier protein (ACP) domains shuttle substrates to catalytically active sites embedded in the chamber wall. We devised synthetic FASs by integrating heterologous enzymes into the reaction chambers and demonstrated their capability to convert acyl-ACP or acyl-CoA from canonical fatty acid biosynthesis to short/medium-chain fatty acids and methyl ketones.

PMID:
28218911
DOI:
10.1038/nchembio.2301
[Indexed for MEDLINE]

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