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Nat Immunol. 2017 Apr;18(4):393-401. doi: 10.1038/ni.3686. Epub 2017 Feb 20.

Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection.

Author information

1
Institute of Immunology, Hannover Medical School, Hannover, Germany.
2
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation, Hannover Medical School, Hannover, Germany.
3
Integrated Research and Treatment Center Transplantation, Hannover Medical School, Hannover, Germany.
4
Genome Analytics, Helmholtz Centre for Infection Research, Braunschweig, Germany.
5
Department Obstetrics, Gynecology and Reproductive Medicine, Hannover Medical School, Hannover, Germany.

Abstract

To investigate how the human γδ T cell pool is shaped during ontogeny and how it is regenerated after transplantation of hematopoietic stem cells (HSCs), we applied an RNA-based next-generation sequencing approach to monitor the dynamics of the repertoires of γδ T cell antigen receptors (TCRs) before and after transplantation in a prospective cohort study. We found that repertoires of rearranged genes encoding γδ TCRs (TRG and TRD) in the peripheral blood of healthy adults were stable over time. Although a large fraction of human TRG repertoires consisted of public sequences, the TRD repertoires were private. In patients undergoing HSC transplantation, γδ T cells were quickly reconstituted; however, they had profoundly altered TCR repertoires. Notably, the clonal proliferation of individual virus-reactive γδ TCR sequences in patients with reactivation of cytomegalovirus revealed strong evidence for adaptive anti-viral γδ T cell immune responses.

PMID:
28218745
DOI:
10.1038/ni.3686
[Indexed for MEDLINE]

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