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Sci Rep. 2017 Feb 20;7:42918. doi: 10.1038/srep42918.

Interaction of Cutibacterium ( formerly Propionibacterium) acnes with bone cells: a step toward understanding bone and joint infection development.

Author information

1
EA3826, Laboratory of Clinical and Experimental Therapeutics of Infections, IRS2, 22 Bd Benoni-Goullin, University of Nantes, Nantes, France.
2
Bacteriology and Hygiene Unit, CHU NANTES, France.
3
INSERM, UMR 957, Pathophysiology of Bone Resorption Laboratory and Therapy of Primary Bone Tumors, Medicine School, University of Nantes, Nantes, France.
4
CHU NANTES, Hôtel Dieu, Nantes, France.
5
INSERM, U1047, University of Montpellier, Nîmes, France.
6
Department of Microbiology, Caremeau University Hospital, Nîmes, France.
7
Clinique chirurgicale orthopédique et traumatique, CHU NANTES, Nantes, France.
8
Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, UK.
9
CRCINA, INSERM, Université d'Angers, Université de Nantes Nantes, France.

Abstract

Cutibacterium acnes (formerly Propionibacterium acnes) is recognized as a pathogen in foreign-body infections (arthroplasty or spinal instrumentation). To date, the direct impact of C. acnes on bone cells has never been explored. The clade of 11 C. acnes clinical isolates was determined by MLST. Human osteoblasts and osteoclasts were infected by live C. acnes. The whole genome sequence of six isolates of this collection was analyzed. CC36 C. acnes strains were significantly less internalized by osteoblasts and osteoclasts than CC18 and CC28 C. acnes strains (p ≤ 0.05). The CC18 C. acnes ATCC6919 isolate could survive intracellularly for at least 96 hours. C. acnes significantly decreased the resorption ability of osteoclasts with a major impact by the CC36 strain (p ≤ 0.05). Genome analysis revealed 27 genes possibly linked to these phenotypic behaviors. We showed a direct impact of C. acnes on bone cells, providing new explanations about the development of C. acnes foreign-body infections.

PMID:
28218305
PMCID:
PMC5317161
DOI:
10.1038/srep42918
[Indexed for MEDLINE]
Free PMC Article

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