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Microb Biotechnol. 2017 May;10(3):604-611. doi: 10.1111/1751-7915.12697. Epub 2017 Feb 20.

Evaluation of Rv0220, Rv2958c, Rv2994 and Rv3347c of Mycobacterium tuberculosis for serodiagnosis of tuberculosis.

You X1,2,3,4, Li R1,2,3, Wan K5, Liu L1,2,3, Xie X6, Zhao L1,2,3, Wu N4, Deng X1,2,3, Wang L1,2,3, Zeng Y1,2,3.

Author information

Institute of Pathogenic Biology, Medical College, University of South China, Hengyang, 421001, China.
Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang, 421001, China.
Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, 421001, China.
Clinical laboratory, The First Affiliated Hospital of University of South China, Hengyang, 421000, China.
State Key Laboratory for Infectious Disease Prevention and Control/National Institute for communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Clinical laboratory, Hengyang No.1 People's Hospital, Hengyang, 421001, China.

Erratum in


Tuberculosis (TB), the leading cause of death among infectious diseases worldwide, is caused by Mycobacterium tuberculosis (M. tuberculosis). Early accurate diagnosis means earlier prevention, treatment and control of TB. To confirm efficient diagnostic antigens for M. tuberculosis, the serodiagnosis value of four recombinant proteins including Rv0220, Rv2958c, Rv2994 and Rv3347c was evaluated in this study. The specificities and sensitivities of four recombinant proteins were determined based on enzyme-linked immunosorbent assay (ELISA) by screening sera from smear-positive pulmonary TB patients (n = 92), uninfected individuals (n = 60) and patients with Mycoplasma pneumoniae (n = 32) that potentially cross-react with M. tuberculosis. The ELISAs showed that Rv0220, Rv2958c, Rv2994 and Rv3347c exhibited high specificities and sensitivities in detecting immunoglobulin G (IgG) antibody, with 98.3/91.3%, 91.7/85.9%, 93.3/89.1% and 93.3/80.4% respectively. According to the receiver-operating characteristic (ROC) analysis, the area under the ROC of the target proteins was 0.988, 0.969, 0.929 and 0.945 respectively. Western blot was established to evaluate the immunoreactivities of target proteins to mice and human sera. Results demonstrated that Rv0220, Rv2958c, Rv2994 and Rv3347c could specifically recognize TB-positive sera and the sera of mice immunized with the corresponding protein. Thus, Rv0220, Rv2958c, Rv2994 and Rv3347c were valuable potential diagnostic antigens for M. tuberculosis.

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