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Life Sci. 2017 Mar 15;173:68-72. doi: 10.1016/j.lfs.2017.02.006. Epub 2017 Feb 14.

Pharmacological postconditioning with sevoflurane activates PI3K/AKT signaling and attenuates cardiopulmonary bypass-induced lung injury in dog.

Author information

1
Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China.
2
Guizhou key laboratory of anesthesia and organ protection, Zunyi Medical College, Zunyi 563000, China.
3
Department of Anesthesiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563000, China. Electronic address: 420557377@qq.com.

Abstract

AIMS:

Sevoflurane, a widely used inhaled anesthetic, showed valid protective effect against ischemia reperfusion injury in lung, however, the underlying molecular mechanisms has yet to be explained.

MATERIALS AND METHODS:

Dogs were divided into four groups: control group, cardiopulmonary bypass (CPB) group, sevoflurane postconditioning group and wortmannin group. Arterial blood was obtained from each group to test the respiratory index and oxygenation index and pulmonary tissues were sampled for apoptotic TUNEL stain. Western Blot was performed to check the level of Akt, p-Akt and nuclear factor kappa B (NF-κB), while ELISA was performed to determine the protein concentrations of tumor necrosis factor α (TNF-α).

KEY FINDINGS:

Pharmacological postconditioning with sevoflurane protected dog lung from I/R injury with CPB, improved pulmonary dysfunction, and decreased pneumonocyte apoptosis. Application of wortmannin, a specific inhibitor of PI3K, abolished the protective effects and anti-apoptotic effects of sevoflurane-postcondition. Wortmannin also inhibited the sevoflurane-postcondition-induced phosphorylation of AKT and up-regulated the level of NF-κB and TNF-α.

SIGNIFICANCE:

Accordingly, present study demonstrated that PI3K/AKT/NF-κB pathway plays an important role in the protective effect of sevoflurane postconditioning against CPB induced lung injury.

KEYWORDS:

Cardiopulmonary bypass; Ischemia-reperfusion injury; Pulmonary; Sevoflurane

PMID:
28212826
DOI:
10.1016/j.lfs.2017.02.006
[Indexed for MEDLINE]

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