Oxyresveratrol Supplementation to C57bl/6 Mice Fed with a High-Fat Diet Ameliorates Obesity-Associated Symptoms

Nutrients. 2017 Feb 16;9(2):147. doi: 10.3390/nu9020147.

Abstract

Oxyresveratrol has been proven effective in inhibiting adipogenesis in a 3T3-L1 cell model. We investigated the preventive effect of oxyresveratrol supplementation on obesity development in high-fat diet-fed mice. Male C57bl/6 mice were randomly subjected to control (5% fat by weight, LF), high-fat (30% fat by weight, HF), and high-fat supplemented with 0.25% and 0.5% oxyresveratrol (OXY1 and OXY2, respectively) diet groups for eight weeks. Oxyresveratrol supplementation effectively alleviated obesity-associated symptoms such as insulin resistance, hyperglycemia, and hepatic steatosis in high-fat diet-fed mice. Compared to the high-fat diet group, oxyresveratrol supplementation suppressed expression of glucose-6-phosphatase, sterol regulatory element-binding proteins 1, fatty acid synthase and CCAAT/Enhancer-binding proteins α, and elevated AMP-activated protein kinase (α2-catalytic subunit) level in liver, upregulated insulin-dependent glucose transporter type 4 level in adipose tissue, and increased expression of insulin receptor substrate 1, insulin-dependent glucose transporter type 4, AMP-activated protein kinase α, peroxisome proliferator-activated receptor γ coactivator-1α, and sirtuin 1 in muscle to regulate lipid and glucose homeostasis in these tissues. This study demonstrated that oxyresveratrol supplementation effectively ameliorated obesity-associated symptoms in high-fat diet-fed mice, presumably attributed to mediating critical regulators involved in lipid and glucose homeostasis in liver, visceral fat, and muscle.

Keywords: amelioration; glucose homeostasis; high-fat diet; lipid homeostasis; obesity; oxyresveratrol supplementation.

MeSH terms

  • Adipogenesis / drug effects
  • Animals
  • Diet, High-Fat / adverse effects*
  • Dietary Supplements
  • Fatty Liver / prevention & control
  • Glucose / metabolism
  • Homeostasis / drug effects
  • Hyperglycemia / prevention & control
  • Insulin Resistance
  • Intra-Abdominal Fat / metabolism
  • Lipid Metabolism / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Muscles / metabolism
  • Obesity / prevention & control*
  • Plant Extracts / administration & dosage*
  • Stilbenes / administration & dosage*

Substances

  • Plant Extracts
  • Stilbenes
  • puag-haad
  • Glucose