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Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.

A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants.

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Department of Biotechnology, School of Life Science and Technology, Centre for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Department of Biology, East Carolina University, Greenville, North Carolina 27834, USA.
Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Centre for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009, China.
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland 20742, USA.
Department of Genetics, Cell Biology &Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455, USA.
Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, Maryland 20850, USA.


Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 generated biallelic mutations at nearly 100% efficiency at four independent sites in rice T0 transgenic plants. Moreover, we repurposed AsCpf1 and LbCpf1 for efficient transcriptional repression in Arabidopsis, and demonstrated a more than tenfold reduction in miR159b transcription. Our data suggest promising applications of CRISPR-Cpf1 for editing plant genomes and modulating the plant transcriptome.

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