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Science. 2017 Feb 24;355(6327). pii: eaah6317. doi: 10.1126/science.aah6317. Epub 2017 Feb 16.

Mechanisms for initiating cellular DNA replication.

Author information

1
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. franziska.bleichert@fmi.ch mbotchan@berkeley.edu jmberger@jhmi.edu.
2
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA. franziska.bleichert@fmi.ch mbotchan@berkeley.edu jmberger@jhmi.edu.

Abstract

Cellular DNA replication factories depend on ring-shaped hexameric helicases to aid DNA synthesis by processively unzipping the parental DNA helix. Replicative helicases are loaded onto DNA by dedicated initiator, loader, and accessory proteins during the initiation of DNA replication in a tightly regulated, multistep process. We discuss here the molecular choreography of DNA replication initiation across the three domains of life, highlighting similarities and differences in the strategies used to deposit replicative helicases onto DNA and to melt the DNA helix in preparation for replisome assembly. Although initiators and loaders are phylogenetically related, the mechanisms they use for accomplishing similar tasks have diverged considerably and in an unpredictable manner.

PMID:
28209641
DOI:
10.1126/science.aah6317
[Indexed for MEDLINE]

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