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Arch Pharm Res. 2017 Mar;40(3):364-372. doi: 10.1007/s12272-017-0895-0. Epub 2017 Feb 15.

Determination of urazamide in pharmaceutical preparation with room temperature ionic liquid.

Author information

1
College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea.
2
College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea.
3
College of Pharmacy, Kangwon National University, Chuncheon, 24341, Korea. kyeong@kangwon.ac.kr.

Abstract

A high performance liquid chromatographic method was developed and validated for the determination of urazamide in pharmaceutical preparation with novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs). 1-Ethyl-3-methyl-imidazolium tetrafluoroborate ([EMIM][BF4]) was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t0. Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine urazamide at the proper retention time. The assay was validated according to International Conference on Harmonization guidelines. The linearity of the calibration curve was good (r2 > 0.999). Intra-day precision varied between 0.50 and 1.23%. Relative standard deviations of inter-day precision ranged between 1.07 and 1.66%. Recoveries in tablets ranged between 99.7 and 101.2% and it was successfully applied to determine urazamide in pharmaceutical preparations.

KEYWORDS:

Reverse phase high performance liquid chromatography (RP-HPLC); Room temperature ionic liquids (RTILs); Urazamide; Very polar compound

PMID:
28205012
DOI:
10.1007/s12272-017-0895-0
[Indexed for MEDLINE]

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