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PLoS Negl Trop Dis. 2017 Feb 15;11(2):e0005313. doi: 10.1371/journal.pntd.0005313. eCollection 2017 Feb.

Comparative and functional triatomine genomics reveals reductions and expansions in insecticide resistance-related gene families.

Author information

1
Laboratorio de Neurobiología de Insectos, Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina.
2
Centro de Bioinvestigaciones (CeBio) and Centro de Investigación y Transferencia del Noroeste de Buenos Aires (CITNOBA-CONICET), Universidad Nacional del Noroeste de la Provincia de Buenos Aires, Pergamino, Argentina.
3
Instituto Nacional de Salud Pública, Cuernavaca, México.
4
Centro de Referencia de Vectores (CeReVe), Dirección de Enfermedades Transmisibles por Vectores, Ministerio de Salud de la Nación Argentina, Santa María de Punilla, Córdoba, Argentina.
5
Centro de Endocrinología Aplicada y Experimental, Facultad de Medicina, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
6
Grupo de Comportamento de Vetores e Interação com Patógenos-CNPq, Centro de Pesquisas René Rachou-FIOCRUZ, Belo Horizonte, Brazil.

Abstract

BACKGROUND:

Triatomine insects are vectors of Trypanosoma cruzi, a protozoan parasite that is the causative agent of Chagas' disease. This is a neglected disease affecting approximately 8 million people in Latin America. The existence of diverse pyrethroid resistant populations of at least two species demonstrates the potential of triatomines to develop high levels of insecticide resistance. Therefore, the incorporation of strategies for resistance management is a main concern for vector control programs. Three enzymatic superfamilies are thought to mediate xenobiotic detoxification and resistance: Glutathione Transferases (GSTs), Cytochromes P450 (CYPs) and Carboxyl/Cholinesterases (CCEs). Improving our knowledge of key triatomine detoxification enzymes will strengthen our understanding of insecticide resistance processes in vectors of Chagas' disease.

METHODS AND FINDINGS:

The discovery and description of detoxification gene superfamilies in normalized transcriptomes of three triatomine species: Triatoma dimidiata, Triatoma infestans and Triatoma pallidipennis is presented. Furthermore, a comparative analysis of these superfamilies among the triatomine transcriptomes and the genome of Rhodnius prolixus, also a triatomine vector of Chagas' disease, and other well-studied insect genomes was performed. The expression pattern of detoxification genes in R. prolixus transcriptomes from key organs was analyzed. The comparisons reveal gene expansions in Sigma class GSTs, CYP3 in CYP superfamily and clade E in CCE superfamily. Moreover, several CYP families identified in these triatomines have not yet been described in other insects. Conversely, several groups of insecticide resistance related enzymes within each enzyme superfamily are reduced or lacking in triatomines. Furthermore, our qRT-PCR results showed an increase in the expression of a CYP4 gene in a T. infestans population resistant to pyrethroids. These results could point to an involvement of metabolic detoxification mechanisms on the high levels of pyrethroid resistance detected in triatomines from the Gran Chaco ecoregion.

CONCLUSIONS AND SIGNIFICANCE:

Our results help to elucidate the potential insecticide resistance mechanisms in vectors of Chagas' disease and provide new relevant information for this field. This study shows that metabolic resistance might be a contributing cause of the high pyrethroid resistance observed in wild T. infestans populations from the Gran Chaco ecoregion, area in which although subjected to intense pyrethroid treatments, vector control has failed. This study opens new avenues for further functional studies on triatomine detoxification mechanisms.

PMID:
28199333
PMCID:
PMC5310753
DOI:
10.1371/journal.pntd.0005313
[Indexed for MEDLINE]
Free PMC Article

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