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Oncoimmunology. 2016 Nov 18;6(1):e1258505. doi: 10.1080/2162402X.2016.1258505. eCollection 2017.

Caspase-2 and oxidative stress underlie the immunogenic potential of high hydrostatic pressure-induced cancer cell death.

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Sotio, Prague, Czech Republic; 2nd Medical Faculty and University Hospital Motol, Charles University, Prague, Czech Republic.
Cell Death Research and Therapy Unit, Department of Cellular and Molecular Medicine, KU Leuven University of Leuven , Leuven, Belgium.
Laboratory of Tumor Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic , Prague, Czech Republic.
Centre de Recherche en Cancérologie Nantes-Angers, INSERM, U892 , Nantes, France.
Sotio , Prague, Czech Republic.


High hydrostatic pressure (HHP) promotes key characteristics of immunogenic cell death (ICD), in thus far resembling immunogenic chemotherapy and ionizing irradiation. Here, we demonstrate that cancer cells succumbing to HHP induce CD4+ and CD8+ T cell-dependent protective immunity in vivo. Moreover, we show that cell death induction by HHP relies on the overproduction of reactive oxygen species (ROS), causing rapid establishment of the integrated stress response, eIF2α phosphorylation by PERK, and sequential caspase-2, -8 and -3 activation. Non-phosphorylatable eIF2α, depletion of PERK, caspase-2 or -8 compromised calreticulin exposure by cancer cells succumbing to HHP but could not inhibit death. Interestingly, the phagocytosis of HHP-treated malignant cells by dendritic cells was suppressed by the knockdown of caspase-2 in the former. Thus, caspase-2 mediates a key function in the interaction between dying cancer cells and antigen presenting cells. Our results indicate that the ROS→PERK→eIF2α→caspase-2 signaling pathway is central for the perception of HHP-driven cell death as immunogenic.


Caspases; ER stress; ecto-CALR; high hydrostatic pressure; immunogenic cell death

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