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Nitric Oxide. 2017 Aug 1;68:125-136. doi: 10.1016/j.niox.2017.02.004. Epub 2017 Feb 11.

Post-translational modifications of Medicago truncatula glutathione peroxidase 1 induced by nitric oxide.

Author information

1
UMR INRA 1355, CNRS 7254, Université Nice Sophia Antipolis, Institut Sophia Agrobiotech, 06903 Sophia Antipolis Cedex, France.
2
Department of Biochemistry & Biotechnology, Laboratory of Plant & Environmental Biotechnology, University of Thessaly, Biopolis, 41500 Larissa, Greece.
3
IRHS, Université d'Angers, INRA, AGROCAMPUS-Ouest, SFR 4207 QUASAV, 42 rue Georges Morel, 49071 Beaucouzé cedex, France.
4
UMR 1136 Interactions Arbres-Microorganismes, Université de Lorraine/INRA, F-54500 Vandoeuvre-lès-Nancy, France.
5
UMR INRA 1355, CNRS 7254, Université Nice Sophia Antipolis, Institut Sophia Agrobiotech, 06903 Sophia Antipolis Cedex, France. Electronic address: renaud.brouquisse@inra.fr.

Abstract

Plant glutathione peroxidases (Gpx) catalyse the reduction of various peroxides, such as hydrogen peroxide (H2O2), phospholipid hydroperoxides and peroxynitrite, but at the expense of thioredoxins rather than glutathione. A main function of plant Gpxs is the protection of biological membranes by scavenging phospholipid hydroperoxides, but some Gpxs have also been associated with H2O2 sensing and redox signal transduction. Nitric oxide (NO) is not only known to induce the expression of Gpx family members, but also to inhibit Gpx activity, presumably through the S-nitrosylation of conserved cysteine residues. In the present study, the effects of NO-donors on both the activity and S-nitrosylation state of purified Medicago truncatula Gpx1 were analyzed using biochemical assay measurements and a biotin-switch/mass spectrometry approach. MtGpx1 activity was only moderately inhibited by the NO-donors diethylamine-NONOate and S-nitrosoglutathione, and the inhibition may be reversed by DTT. The three conserved Cys of MtGpx1 were found to be modified through S-nitrosylation and S-glutathionylation, although to different extents, by diethylamine-NONOate and S-nitrosoglutathione, or by a combination of diethylamine-NONOate and reduced glutathione. The regulation of MtGpx1 and its possible involvement in the signaling process is discussed in the light of these results.

KEYWORDS:

Glutathione peroxidase; Medicago truncatula; Nitric oxide; S-glutathionylation; S-nitrosylation

PMID:
28193486
DOI:
10.1016/j.niox.2017.02.004
[Indexed for MEDLINE]

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