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BMC Genomics. 2017 Feb 13;18(1):152. doi: 10.1186/s12864-017-3582-0.

Genome-wide characterization of JASMONATE-ZIM DOMAIN transcription repressors in wheat (Triticum aestivum L.).

Wang Y1,2, Qiao L3, Bai J1,2, Wang P1,2,4, Duan W1,2,5, Yuan S1,2, Yuan G1,2, Zhang F1,2, Zhang L6,7, Zhao C8,9.

Author information

1
Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China.
2
The Municipal Key Laboratory of Molecular Genetics for Hybrid Wheat, Beijing, 100097, China.
3
Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, China.
4
College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
5
College of Life Sciences, Capital Normal University, Beijing, 100048, China.
6
Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China. lpzhang8@126.com.
7
The Municipal Key Laboratory of Molecular Genetics for Hybrid Wheat, Beijing, 100097, China. lpzhang8@126.com.
8
Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China. cp_zhao@vip.sohu.com.
9
The Municipal Key Laboratory of Molecular Genetics for Hybrid Wheat, Beijing, 100097, China. cp_zhao@vip.sohu.com.

Abstract

BACKGROUND:

The JASMONATE-ZIM DOMAIN (JAZ) repressor family proteins are jasmonate co-receptors and transcriptional repressor in jasmonic acid (JA) signaling pathway, and they play important roles in regulating the growth and development of plants. Recently, more and more researches on JAZ gene family are reported in many plants. Although the genome sequencing of common wheat (Triticum aestivum L.) and its relatives is complete, our knowledge about this gene family remains vacant.

RESULTS:

Fourteen JAZ genes were identified in the wheat genome. Structural analysis revealed that the TaJAZ proteins in wheat were as conserved as those in other plants, but had structural characteristics. By phylogenetic analysis, all JAZ proteins from wheat and other plants were clustered into 11 sub-groups (G1-G11), and TaJAZ proteins shared a high degree of similarity with some JAZ proteins from Aegliops tauschii, Brachypodium distachyon and Oryza sativa. The Ka/Ks ratios of TaJAZ genes ranged from 0.0016 to 0.6973, suggesting that the TaJAZ family had undergone purifying selection in wheat. Gene expression patterns obtained by quantitative real-time PCR (qRT-PCR) revealed differential temporal and spatial regulation of TaJAZ genes under multifarious abiotic stress treatments of high salinity, drought, cold and phytohormone. Among these, TaJAZ7, 8 and 12 were specifically expressed in the anther tissues of the thermosensitive genic male sterile (TGMS) wheat line BS366 and normal control wheat line Jing411. Compared with the gene expression patterns in the normal wheat line Jing411, TaJAZ7, 8 and 12 had different expression patterns in abnormally dehiscent anthers of BS366 at the heading stage 6, suggesting that specific up- or down-regulation of these genes might be associated with the abnormal anther dehiscence in TGMS wheat line.

CONCLUSION:

This study analyzed the size and composition of the JAZ gene family in wheat, and investigated stress responsive and differential tissue-specific expression profiles of each TaJAZ gene in TGMS wheat line BS366. In addition, we isolated 3 TaJAZ genes that would be more likely to be involved in the regulation of abnormal anther dehiscence in TGMS wheat line. In conclusion, the results of this study contributed some novel and detailed information about JAZ gene family in wheat, and also provided 3 potential candidate genes for improving the TGMS wheat line.

KEYWORDS:

Anther dehiscence; Gene expression; JASMONATE-ZIM DOMAIN; Phylogenetic analysis; Thermo sensitive genic male sterile (TGMS)

PMID:
28193162
PMCID:
PMC5307646
DOI:
10.1186/s12864-017-3582-0
[Indexed for MEDLINE]
Free PMC Article

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