Format

Send to

Choose Destination
Nat Methods. 2017 Feb 13;14(4):399-402. doi: 10.1038/nmeth.4178.

Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.

Author information

1
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, USA.
2
Howard Hughes Medical Institute, UCLA-DOE Institute, Departments of Biological Chemistry, Chemistry &Biochemistry, and Molecular Biology Institute, UCLA, Los Angeles, California, USA.
3
Department of Structural Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Abstract

Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.

PMID:
28192420
PMCID:
PMC5376236
DOI:
10.1038/nmeth.4178
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center