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Nat Methods. 2017 Apr;14(4):395-398. doi: 10.1038/nmeth.4179. Epub 2017 Feb 13.

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput.

Author information

1
Koch Institute for Integrative Cancer Research, MIT, Cambridge, Massachusetts, USA.
2
Institute for Medical Engineering &Science (IMES) and Department of Chemistry, MIT, Cambridge, Massachusetts, USA.
3
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
4
Ragon Institute of MGH, MIT and Harvard, Cambridge, Massachusetts, USA.
5
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA.
6
Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York, USA.
7
New York Genome Center, New York, New York, USA.

Abstract

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

PMID:
28192419
PMCID:
PMC5376227
DOI:
10.1038/nmeth.4179
[Indexed for MEDLINE]
Free PMC Article

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