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J Allergy Clin Immunol. 2017 Jun;139(6):1885-1896.e9. doi: 10.1016/j.jaci.2016.12.971. Epub 2017 Feb 10.

Systemic innate immune activation in food protein-induced enterocolitis syndrome.

Author information

1
Jaffe Food Allergy Institute, New York, NY.
2
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY.
3
Human Immune Monitoring Core, Icahn School of Medicine at Mount Sinai, New York, NY.
4
Jaffe Food Allergy Institute, New York, NY. Electronic address: cecilia.berin@mssm.edu.

Abstract

BACKGROUND:

Food protein-induced enterocolitis syndrome (FPIES) is a non-IgE-mediated food allergy of infancy whose pathophysiology is poorly understood.

OBJECTIVES:

We set out to identify and phenotype allergen-responsive cells in peripheral blood of a cohort of subjects undergoing supervised food challenge for FPIES.

METHODS:

We profiled antigen-responsive cells in PBMCs by flow cytometry, and examined cells in whole blood obtained before and after challenge by CyTOF mass cytometry and RNAseq.

RESULTS:

Using a CD154-based detection approach, we observed that milk, soy, or rice-responsive T cells, and TNF-α-producing CD154+ T cells, were significantly lower in those with outgrown FPIES compared with those with active FPIES. However, levels were within the normal range and were inconsistent with a role in the pathophysiology of FPIES. Profiling of whole blood by CyTOF demonstrated profound activation of cells of the innate immune system after food challenge, including monocytes, neutrophils, natural killer cells, and eosinophils. Activation was not observed in children with outgrown FPIES. We confirmed this pattern of innate immune activation in a larger cohort by RNAseq. Furthermore, we observed pan-T-cell activation and redistribution from the circulation after a positive food challenge but not in those who had outgrown their FPIES.

CONCLUSIONS:

Our data demonstrate a compelling role of systemic innate immune activation in adverse reactions elicited by foods in FPIES. Further investigation is needed to identify the mechanism of antigen specificity of adverse reactions to foods in FPIES.

KEYWORDS:

CyTOF; FPIES; Food allergy; RNA-sequencing; innate; non-IgE

PMID:
28192147
PMCID:
PMC5461215
DOI:
10.1016/j.jaci.2016.12.971
[Indexed for MEDLINE]
Free PMC Article

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