Format

Send to

Choose Destination
J Toxicol Pathol. 2017 Jan;30(1):79-107. doi: 10.1293/tox.2016-0047. Epub 2016 Sep 26.

Databases for technical aspects of immunohistochemistry.

Author information

1
Conference on Experimental Animal Histopathology; Biological Research Laboratories, Nissan Chemical Industries, Ltd., 1470 Shiraoka, Shiraoka-shi, Saitama 349-0294, Japan.
2
Conference on Experimental Animal Histopathology; Regulatory Affairs Division, Biosciences Sales & Marketing, Ishihara Sangyo Kaisha, Ltd., 2-3-1, Nishishibukawa, Kusatsu, Shiga 525-0025, Japan.
3
Conference on Experimental Animal Histopathology; Laboratory of Pathology, Faculty of Pharmaceutical Science, Setsunan University, 45-1 Nagaotohge-cho, Hirakata, Osaka 573-0101, Japan.

Abstract

With the aims of sharing information about the technical aspects of immunohistochemistry (IHC) and making it possible to make a suitable choice of antibody for histopathological examination, this technical report describes the results of a questionnaire administered during the period of 2014 to 2015 to members of the Conference on Experimental Animal Histopathology. It also describes the immunological properties of primary antibodies (clone, supplier, catalog number, species reactivity, etc.) and the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, secondary antibody information, etc.) for a total number of 733 primary antibodies (425 kinds of primary antibody).

KEYWORDS:

antibody; immunohistochemistry; toxicological pathology

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center