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J Clin Virol. 2017 Apr;89:10-13. doi: 10.1016/j.jcv.2017.02.002. Epub 2017 Feb 6.

Isolation of hepatitis E virus genotype 4 from patients with acute cryptogenic hepatitis in Korea.

Author information

1
Department of Internal Medicine, Seoul National University Bundang Hospital, College of Medicine, Seoul National University, Seongnam, Gyeonggi 13620, Republic of Korea.
2
Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
3
Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. Electronic address: ischoi@konkuk.ac.kr.

Abstract

BACKGROUND:

Autochthonous hepatitis E occurs sporadically in developed countries. The consumption of undercooked pork containing hepatitis E virus genotype 3 (HEV-3) or 4 (HEV-4) is the major risk factor for infection. The serological diagnostic kits currently used in hospitals sometimes produce false-negative or -positive results. Therefore, detection of both HEV RNA and antibodies to the virus is required for confirmative diagnosis of hepatitis E.

OBJECTIVES:

We aimed to detect HEV in serum samples from patients with cryptogenic hepatitis and to determine the origin of HEV.

STUDY DESIGN:

A nested polymerase chain reaction (PCR) method was developed for detection of HEV-3 and HEV-4 in patients with hepatitis. A total of 23 serum samples, deposited in 2006-2012, from patients with acute cryptogenic hepatitis who were serologically negative for hepatitis A, B, C, and E were examined using this method. The amplified PCR products were genetically analyzed.

RESULTS:

Four HEV-4 isolates were detected from the 23 serum samples. Phylogenetic analysis indicated that three of the four isolates were closely related to HEV-4 isolates found in pigs in Korea and in patients with hepatitis E in Japan.

CONCLUSIONS:

The newly developed nested PCR method was useful for detection of HEV in patients with cryptogenic hepatitis. The close relationship between the human HEV-4 isolates identified in this study and swine isolates implied that zoonotic transmission of HEV might be a source of infection in patients with hepatitis.

KEYWORDS:

Cryptogenic; Hepatitis; Hepatitis e virus genotype 4; Korea; Pigs

PMID:
28189935
DOI:
10.1016/j.jcv.2017.02.002
[Indexed for MEDLINE]

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