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Int J Pharm. 2017 Apr 15;521(1-2):184-195. doi: 10.1016/j.ijpharm.2017.01.069. Epub 2017 Feb 7.

Laser deposition of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - lysozyme microspheres based coatings with anti-microbial properties.

Author information

1
National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania; Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, Bucharest 011061, Romania.
2
Politehnica University of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, Polizu Street no 1-7, Bucharest 011061, Romania; University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, Bucharest 060101, Romania.
3
Institute of Biochemistry, Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, Romania.
4
Research Institute of the University of Bucharest - ICUB, Bucharest, Romania.
5
Institute of Cellular Biology and Pathology "N. Simionescu", Proteomics Department, B. P. Hasdeu Street, No. 8, 050568 Bucharest, Romania.
6
National Institute of Materials Physics, Magurele, Ilfov, Romania.
7
National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania.
8
University of Bucharest, Faculty of Biology, Microbiology Immunology Department, Aleea Portocalelor no 1-3, Bucharest 060101, Romania.
9
National Institute for Lasers, Plasma & Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Bucharest 769231, Romania. Electronic address: gabriel.socol@inflpr.o.

Abstract

The purpose of this study was to obtain, characterize and evaluate the cytotoxicity and antimicrobial activity of coatings based on poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) - Lysozyme (P(3HB-3HV)/Lys) and P(3HB-3HV) - Polyethylene glycol - Lysozyme (P(3HB-3HV)/PEG/Lys) spheres prepared by Matrix Assisted Pulsed Laser Evaporation (MAPLE) technique, in order to obtain functional and improved Ti-based implants. Morphological investigation of the coatings by Infrared Microscopy (IRM) and SEM revealed that the average diameter of P(3HB-3HV)/Lys spheres is around 2μm and unlike the drop cast samples, IRM recorded on MAPLE films revealed a good distribution of monitored functional groups on the entire scanned surface. The biological evaluation of MAPLE structured surfaces revealed an improved biocompatibility with respect to osteoblasts and endothelial cells as compared with Ti substrates and an enhanced anti-biofilm effect against Gram positive (Staphylococcus aureus) and Gram negative (Pseudomonas aeruginosa) tested strains. Thus, we propose that the fabricated P(3HB-3HV)/PEG/Lys and P(3HB-3HV)/Lys microspheres may be efficiently used as a matrix for controlled local drug delivery, with practical applications in developing improved medical surfaces for the reduction of implant-associated infections.

KEYWORDS:

Antimicrobial surfaces; Laser processing; Lysozyme; MAPLE thin coatings; Osteoblast and endothelial cell culture

PMID:
28188877
DOI:
10.1016/j.ijpharm.2017.01.069
[Indexed for MEDLINE]

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