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Surg Obes Relat Dis. 2017 Apr;13(4):661-668. doi: 10.1016/j.soard.2016.10.024. Epub 2016 Dec 20.

Bariatric surgery attenuates colitis in an obese murine model.

Author information

1
Department of Surgery, University of California, Irvine, CA.
2
Microbiology and Molecular Genetics, University of California, Irvine, CA.
3
Department of Pharmaceutical Sciences, West Coast University, Los Angeles, CA.
4
Department of Pathology & Laboratory Medicine, University of California, Irvine, CA.
5
Department of Surgery, University of California, Irvine, CA. Electronic address: apigazzi@uci.edu.

Abstract

BACKGROUND:

Obesity and inflammatory bowel disease (IBD) represent chronic inflammatory conditions. Bariatric surgery improves some obesity-related co-morbidities, but the effects of bariatric surgery on IBD have not been well studied.

OBJECTIVES:

To examine if bariatric surgery may attenuate colitis in an obese murine model of IBD and study the mechanisms underlying the postsurgical amelioration of intestinal inflammation.

SETTING:

University of California Irvine, Department of Surgery and Microbiology laboratories.

METHODS:

Obese mice were assigned to one of 2 bariatric procedures [Duodenojejunal Bypass (DJB n = 6), Sleeve Gastrectomy (SG n = 8)]. Sham-operated mice were (Sham n = 8) were used as a control. After recovering from surgery, IBD was induced by administration of 2% dextran sodium sulfate. Fecal samples were collected before and after IBD induction for microbiome analysis. Pathologic analyses and immunohistochemical staining were performed on colon.

RESULTS:

Survival after DJB and SG was higher relative to Sham mice. Histologically, DJB mice had significantly less intestinal inflammation. The observed improvements were not related to a difference in weight among the groups. Farnesoid X receptor staining in the colon was observed quantitatively more in DJB than in SG and sham mice. A statistically significant increase in the number of Lactobacillales was observed in the stool of mice after DJB.

CONCLUSION:

These results suggest that bariatric surgery, in particular DJB, reduces the severity of colitis in a chemically-induced IBD murine model. The anticolitis effects of DJB may be associated with Farnesoid X receptor regulation and gut microbiome rearrangements.

KEYWORDS:

Colitis; Inflammation; Metabolic/Bariatric surgery; Obese murine model; Obesity

PMID:
28185763
DOI:
10.1016/j.soard.2016.10.024
[Indexed for MEDLINE]

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