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J Forensic Sci. 2017 Sep;62(5):1339-1344. doi: 10.1111/1556-4029.13445. Epub 2017 Feb 10.

LIFE BEYOND LIFE - An Easy Way to Derive Lung Fibroblasts from Cadavers.

Author information

1
Emergency Pole, Forensic Medicine Department, Montpellier University Hospital, 371 Avenue du Doyen Gaston Giraud, Montpellier, 34285, France.
2
Molecular Genetics Laboratory - IURC, Montpellier University Hospital, 641 Avenue du Doyen Gaston Giraud, Montpellier, 34093, France.
3
Heart-Lung Pole, Cardiac, Thoracic and Vascular Surgery, Montpellier University Hospital, 375 Avenue du Doyen Gaston Giraud, Montpellier, 34295, France.
4
Heart-Lung Pole, Department of Lung Diseases and Addictology, Montpellier University Hospital, 375 Avenue du Doyen Gaston Giraud, Montpellier, 34295, France.

Abstract

Several protocols have illustrated the possibility of deriving cells, such as fibroblasts, from different organs. These techniques generally concern organs sampled from living persons, but have already been described for cadavers, especially concerning the skin and tendons. We present, for the first time, an easy way to derive pulmonary fibroblasts from a lung tissue sampled from a cadaver and directly culture plated. The fibroblast output was checked daily. We obtained lung fibroblasts from 3 (60%) cadavers and 2 (100%) living persons. The fibroblast output took about 3 days for cells from living persons and took up to 39 days for those from cadavers. We did not clearly identify any parameters that could explain these differences. Nevertheless, these derived cells had the same features as the source cells, especially in terms of morphology and proliferation, and could potentially be used in different research domains such as forensic or regeneration medicine.

KEYWORDS:

explant; fibroblast; forensic autopsy; forensic science; lung; postmortem delay; senescence

PMID:
28185266
DOI:
10.1111/1556-4029.13445
[Indexed for MEDLINE]

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