Format

Send to

Choose Destination
Nucleic Acids Res. 2017 Apr 7;45(6):3395-3406. doi: 10.1093/nar/gkx067.

DNA target recognition domains in the Type I restriction and modification systems of Staphylococcus aureus.

Author information

1
EaStCHEM School of Chemistry, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3FJ, UK.
2
New England Biolabs, 240 County Road, Ipswich, MA 01938-2723, USA.
3
Institute of Infection and Immunity, St George's, University of London, Cranmer Terrace, London, SW17 0RE, UK.
4
Department of Biosciences, Durham University, Stockton Road, Durham, DH1 3LE, UK.

Abstract

Staphylococcus aureus displays a clonal population structure in which horizontal gene transfer between different lineages is extremely rare. This is due, in part, to the presence of a Type I DNA restriction-modification (RM) system given the generic name of Sau1, which maintains different patterns of methylation on specific target sequences on the genomes of different lineages. We have determined the target sequences recognized by the Sau1 Type I RM systems present in a wide range of the most prevalent S. aureus lineages and assigned the sequences recognized to particular target recognition domains within the RM enzymes. We used a range of biochemical assays on purified enzymes and single molecule real-time sequencing on genomic DNA to determine these target sequences and their patterns of methylation. Knowledge of the main target sequences for Sau1 will facilitate the synthesis of new vectors for transformation of the most prevalent lineages of this 'untransformable' bacterium.

PMID:
28180279
PMCID:
PMC5399793
DOI:
10.1093/nar/gkx067
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center