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Genome. 2017 Jun;60(6):485-495. doi: 10.1139/gen-2016-0089. Epub 2017 Jan 4.

Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.).

Author information

1
a Agronomy College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
2
b Biotechnology and Bioscience College, Shenyang Agricultural University, No. 120 Dongling Road, Shenyang, Liaoning 110866, China.
3
c Corn Research Institute, Liaoning Academy of Agricultural Sciences, No. 84 Dongling Road, Shenyang, Liaoning 110866, China.
4
d Laboratory of Plant Bioreactor and Genetics Engineering, Jilin Provincial Key Laboratory of Agricultural Biotechnology, Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences, No. 1363 Shengtai St., Changchun 130033, China.

Abstract

ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.

KEYWORDS:

GUS activity; PCR; ZmSTK2_USP gene; activité GUS; gène ZmSTK2_USP; maize; maïs; pollen-specific promoter; promoteur pollen-spécifique

PMID:
28177828
DOI:
10.1139/gen-2016-0089
[Indexed for MEDLINE]

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