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Anal Biochem. 1989 Sep;181(2):318-20.

Inhibition of the streptavidin-biotin interaction by milk.

Author information

1
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235.

Abstract

Nonfat dried milk is routinely used as a blocking agent and diluent in immunoassays. However, the data presented in this paper demonstrate that milk contains an inhibitor of the biotin-streptavidin interaction. It is recommended that milk be dialyzed or used at lower concentrations when it is employed as a diluent of streptavidin.

PMID:
2817396
DOI:
10.1016/0003-2697(89)90250-9
[Indexed for MEDLINE]

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