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Aging Cell. 2017 Feb;16(1):192-197. doi: 10.1111/acel.12545. Epub 2016 Nov 17.

Robust, universal biomarker assay to detect senescent cells in biological specimens.

Author information

1
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
2
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
3
Laboratory of Cell Proliferation and Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research 'Demokritos', Athens, Greece.
4
Tumor Suppression Group, Molecular Oncology Program, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
5
Molecular and Clinical Cancer Sciences, Manchester Cancer Research Centre, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, UK.
6
Danish Cancer Society Research Center, Copenhagen, Denmark.
7
Science For Life Laboratory, Division of Translational Medicine and Chemical Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
8
Biomedical Research Foundation, Academy of Athens, Athens, Greece.

Abstract

Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.

KEYWORDS:

Sudan Black B; aging; biotin‐linked compounds; immunohistochemistry; senescence

PMID:
28165661
PMCID:
PMC5242262
DOI:
10.1111/acel.12545
[Indexed for MEDLINE]
Free PMC Article

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