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Aging (Albany NY). 2017 Feb 2;9(2):408-418. doi: 10.18632/aging.101167.

Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects.

Author information

1
Institute of Biomedicine (IBIOMED), University of León, León, Spain.
2
Division of Clinical Translational Science, Georgia Prevention Institute, Department of Pediatrics, Augusta University, Augusta, GA, USA.
3
Radiobiology Unit, Laboratory of Molecular and Cellular Biology, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK•CEN), Mol, Belgium.

Abstract

Aging is associated with a decline in autophagy and a state of low-grade inflammation which further affects apoptosis and autophagy. Importantly, these alterations could reverse with regular physical activity. This study assessed the effects of a resistance exercise training program on autophagy, NLRP3 inflammasome, and apoptosis in peripheral blood mononuclear cells (PBMCs) from old subjects. Twenty-six healthy women and men (age, 69.6±1.5 yr) were randomized to a training (TG) or a control (CG) group. TG performed an 8-week resistance training program, while CG followed their daily routines. Protein expression of beclin-1, Atg12, Atg16 and LAMP-2 increased following the training program, while expression of p62/SQSTM1 and phosphorylation of ULK-1 at Ser757 were significantly lower. Resistance exercise also induced a decrease in NLRP3 expression and in the caspase-1/procaspase-1 ratio. Expression of Bcl-2 and Bcl-xL, as well as the Bad/BcL-2 ratio were reduced, and there was a significant decrease in the protein content of caspase-3. The results obtained seem to indicate that 8-week resistance training stimulates autophagy, prevents NLRP3 inflammasome activation, and reduces apoptosis in PBMCs from elderly subjects. These data could have a significant impact in prevention and rehabilitation programs currently employed in elderly population.

KEYWORDS:

apoptosis; autophagy; elderly; inflammasome; resistance exercise

PMID:
28160545
PMCID:
PMC5361672
DOI:
10.18632/aging.101167
[Indexed for MEDLINE]
Free PMC Article

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